A popular internet joke upon the release of Super Mario 64 was "What happened to Super Mario 4 through 63?" If one is to calculate every Mario game released before Super Mario 64, including outliers such as ports and titles not released on Nintendo hardware like Mario Teaches Typing, the game would be the 113th title on the series. However, if you were to only count games released on non-devoted Nintendo hardware and remove ports, the title would be, very anti-climactically, the 63rd Mario game.

The audio used for Bob-ombs, Monty Moles and Goombas in various games starting with Super Mario 64 is actually pieces of Mario's voice saying "Here We Go" from said game after being pitch-shifted. This would continue being the case even after Charles Martinet was replaced with Kevin Afghani starting with Super Mario Bros. Wonder.

The title of "first 3D platformer" is often erroneously given to either 1996's Super Mario 64 or 1995's Jumping Flash!. Despite this, neither game can factually claim that title, with 1990's Alpha Waves being the true owner of the title according to Guinness World Records.

Additionally, 1984's I, Robot - while primarily a shooting game - did feature 3D platforming segments.

In the castle courtyard in Super Mario 64, there is a plaque to a power star with an illegible name written below, often interpreted as either "Eternal Star" or "L is Real 2401" - with the latter being more popular for how it could be seen as a cryptic hint towards unlocking Luigi.

In a poetic coincidence, the source code leak that revealed Luigi's scrapped model would be found 24 years and 1 month after Super Mario 64's release.

In an interview with Satoru Iwata and Shigeru Miyamoto, it was revealed that an early, experimental build of the game had split-screen multiplayer, with Mario and Luigi as playable characters.

The game's planned multiplayer mode ended up being reworked into a Mario Bros.-style minigame thanks to the Nintendo 64's technical limitations making the split-screen concept unfeasible. However, in February of 1996 (just a month before E3 of that year), both multiplayer mode and Luigi were removed, in part because the Nintendo 64 was bundled with only one controller. These statements are corroborated by findings from the Gigaleak, a massive content leak of internal data from Nintendo in 2020 that included early assets from Super Mario 64. Among the leaked material was a model and textures for Luigi and sprites for "1P" and "2P" icons. Luigi's model is dated June 20, 1995, while the icons use the same generic font seen in the Shoshinkai demo from November of that year, rather than the custom font seen in both the E3 1996 demo and the final release.

Although the Headless Snowman is a minor character in his few appearances, he received some publicity in the form of a Nintendo Power joke published around the time of the main circulation of the "L is Real 2401" Super Mario 64 rumors.

In Volume 107 of Nintendo Power, in the April Fool's-themed "April News Briefs" segment, a "Luigi 64" article is present, where Nintendo states that Luigi fans had been campaigning to have the character in his very own game, and the company's response was to create a hoax upcoming game known as "Headless Snowman 64". This game was apparently intended to satisfy fans of the "second bananas" by featuring a cast of both well-known and obscure Nintendo characters, with the obscure characters including the Wave Race 64 announcer, "random audience members from Super Punch-Out!!", "the frogs in the meadow" from Diddy Kong Racing, and the Headless Snowman himself. Additionally, the game would supposedly contain some non-Nintendo characters such as "that bald guy from Blast Corps," and "the bikini woman in Cruis'n USA." Humorously, this never-made game was not going to feature Luigi, with a developer comment at the end of the article confusing him for "one of the civilian women in GoldenEye 007".

In a 1996 interview with Shigeru Miyamoto published in Super Mario 64's first Japanese strategy guides, he revealed that Mario's running animation, speed and movements in that game were influenced by Arale, the main protagonist of the 1980 Akira Toriyama manga/anime "Dr. Slump":


However, Dr. Slump's influence throughout the Mario series could trace back even further. Some particular examples from Mario games include:

• His running speed, animation, the Racoon and Tanooki Suits from Super Mario Bros. 3
• The Wing Cap from Super Mario 64
• The Cat Suit from Super Mario 3D World

Even traits of Mario's happy cartoonish personality, his short stature and gloves, and blue-red-overalls color scheme bear enough similarities that they all could have been taken from Arale's appearances and other elements throughout Dr. Slump. Despite this, outside of Miyamoto's comment, these connections are not known to have been elaborated on by any other employee from Nintendo.

The first-ever footage of Luigi as a playable character in Super Mario 64 was discovered through an old VHS tape in 2023. The footage was captured by a Japanese broadcaster recording footage of Nintendo's 1995 Space World trade show in Japan for a TV program covering the event. Despite this, the footage of Luigi is not actually the primary focus of the clip and as such can barely be seen at all. The video shows Luigi falling back down after performing a spin-jump in what appears to be a red and white test area.

A 2017 study conducted by a team of Canadian neuropsychologists found that playing Super Mario 64 increased grey matter in the brains of older adults, potentially helping to combat dementia and other neurological disorders. According to the study:

According to Shigeru Miyamoto, the design of the Deku Baba enemies in The Legend of Zelda series were a cross between a dog and the giant snapping Clams from Super Mario 64.

Starting with Super Mario 64, the Koopa Troopa's voice is an incredibly short snippet of "HUMAN, YELL - YELL: FEMALE", a sound effect of a woman gleefully screaming from the Best Service Voice Spectral Volume 1 sound pack, modified into different pitches and speeds. In some games, a different snippet from the sound effect is used to represent Koopa Troopas screaming or being frustrated.

Among the files included in the Gigaleak, a massive 2020 content leak of internal data from Nintendo, are assets associated with Super Mario 64 depicting a fully-coded enemy named Motos, which is absent from the final game. The enemy is programed to chase Mario, pick him up, and throw him similarly to Big Bob-omb and Chuckya. Like those characters, Motos can be picked up and thrown, but unlike them, it can only be defeated by being thrown into lava, similarly to the Bully and Big Bully. This weakness implies that Motos was originally intended to appear as an enemy in Lethal Lava Land and Bowser in the Fire Sea, which is corroborated by the fact that various other assets associated with the levels feature "motos" in their filenames.

Further analysis of the source code indicates that Motos were intended to be connected to Bullies, which are labeled "Otos" in the source code and use sound effects that are also associated with Motos. Additionally, the code contains a filename for an "icemotos" miniboss in Snowman's Land, and the "Bully the Bullies" star in Lethal Lava Land is internally named "bigmotos," implying that at least two variants of Motos were conceived as minibosses before being replaced with Big Bully and Chill Bully in the final game.

Additional data in the Gigaleak indicates that at some point in development, Motos was reconfigured into a boss version of the Chuckya enemy, labeled "Katsugikun" in the source code, before being scrapped altogether. Unlike Motos, no assets for Katsugikun exist other than a filename and behavior code.

By standing against the wall between the left and middle paintings for Cool, Cool Mountain and using the first-person view, you can clip the camera through the wall to find what appears to be a large red "F" floating in the void. This is actually part of the entrance to Bowser in the Dark World, a course on the same floor, seen from below.

The game's smoke texture, labelled "gas_b5" in the source code, was intended to be much more detailed in appearance, but ended up corrupted in the final game due to being encoded in the RGBA16 format rather than the intended IA16. While the glitched texture would theoretically look like a swarm of multicolored pixels as a result of this error, it instead appears as a clump of black pixels due to the game displaying it without any colors, resulting in it not looking out of place. Modifying the game's code to convert the texture to the IA16 format results in it properly displaying.

In the European version, the demo clip depicting Mario collecting a ring of coins in Whomp's Fortress sees him grab one less coin than in all other versions of the game, resulting in two coins being left on-screen instead of just one. Oddly, this is the only change made to the game's demo videos between regions.

The skybox of Wet-Dry World, originally believed to be a stock photograph of the hillside town of Casares, Spain, was later found to be a composite of altered photographs of the town of Shibam, Yemen. The sole exception is the red building near the top of the picture, which was identified as the Great Mosque of Muhammad Ali Pasha in the city of Cairo, Egypt. The location fits the idea of Wet-Dry World, because Yemen is a desert country that, like other Middle Eastern countries, experiences sporadic large-scale flash floods.

The game's producer and director Shigeru Miyamoto was asked if he had any references, or anything else he relied on when making all of Mario’s various animations. He responded:

"We tried out a lot of different things using motion capture, but ultimately we ended up doing it all by hand. We created a “skeleton” for Mario that was the basis of his movement."

In the game's files are unused animations for Mario that include:

• Mario putting on his hat faster.
• An alternate animation for when Mario rides a Koopa Troopa shell.
• Mario changing from a free fall to a forward flip.
• Either Mario crying or reaching for something in his pockets.
• Mario's unused running animation
• Mario motionless while sliding
• A forward sideflip.

In the Japanese exclusive Shindou Pak Taiou edition of the game, the famous "backwards long jump" glitch was fixed, as Mario's speed is capped when going backwards. It is the only version of Super Mario 64, barring Super Mario 64 DS and Super Mario 3D All-Stars, with this fix.

The concept of a floating Mario head on the title screen was taken from a prototype of a game called Mario Paint 3D. It is unknown what this game was going to be but it was speculated to have eventually become Mario Artist Paint Studio for the 64DD.