After exiting the elevator on the 12th floor during "On the Job Training" in the Sedgewick Hotel level, the Ghostbusters' television commercial from the first movie can be heard while standing outside of room 1204.

The Rookie in the Realistic Version was modeled after Associate Producer Ryan French.

The likeness and voice of Mayor Jock Mulligan is provided by Brian Doyle Murray, who is also Bill Murray's older brother. Brian Murray was also in the film Ghostbusters II as a different character, the head psychiatrist that spoke to the Ghostbusters after they were arrested towards the end of the film.

The Rookie's likeness was originally modeled after level designer Eric Schatz, but his likeness was reportedly removed because the animators felt his in-game model looked too similar to Gordon Freeman from the Half-Life series.

The original model was only intended to be temporary, as the developers originally planned for the game to include a character creator to let the player determine the appearance of the Rookie. This feature was dropped when they determined that they had to pre-render the game's cinematics.

The faces of the Ghostbusters were not 3D scans of the actors' heads. They were rendered with graphics software using references from still shots from the movie.

The two security guards in the opening movie are modeled after Character Designers Jesse Sosa and Ian McIntosh. Originally, the two security guards were to be modeled after present day Dan Aykroyd and Harold Ramis, hence their names Dan and Harold.

Jason Hawes and Adam Berry from the TV Series Ghost Hunters were originally going to be in the video game, but were removed before the game was released.

On the top floor of the Firehouse in both the PlayStation 3 and Xbox 360 versions of the game is a child's drawing of a Ghostbuster that reads "To Uncle Egon, from Ed". This is likely a reference to the 2007 fan-film: "Return of the Ghostbusters", which stars a new set of Ghostbusters, one of whom is Egon's nephew, named Ed Spengler.

The Proton Pack in the Realistic version of Ghostbusters: The Video Game has a set of blinking lights on the left side of the Blue Chaser Lights. These lights were inspired by Ray's (Dan Aykroyd) Proton Pack from the first Ghostbusters Film.

These lights aren't actually on the Proton Pack, but are a result of the paint on the Proton Pack's shell being chipped away and the adjacent lights shining through. The lights were still added to the game's Proton Pack anyway.

Concept artist Stuart Ng designed a multiplayer level based off of the underground subway from Ghostbusters II, but the level didn't make it into the game.

Early demo footage showed that the Slime Tether attachment was available earlier in the game. Also in the demo footage, a small bubble formed at the tip of the nozzle when the slime tether was selected, which was also left out of the retail version.

Rick Moranis declined to reprise his role as Louis Tully in Ghostbusters: The Video Game because he retired from acting in 1996. He retired from acting to raise his children after losing his wife to cancer.

Although Louis Tully does not appear in the game, his desk can be found at the bottom of the staircase in the firehouse. On the back wall are his uniform and the black earmuffs he wore in Ghostbusters II.

Sigourney Weaver originally declined to reprise her role as Dana Barrett in the video game. When she heard that the original cast was going to return to act in the game, she wanted to be a part of the game. Unfortunately, the game was too far into production to put her back in, so she was left out.

Dan Aykroyd has said in interviews that Ghostbusters: The Video Game is technically the third Ghostbusters movie.

There was a level cut from the final release of the game where the Ghostbusters fight haunted floats in the Thanksgiving Day Parade. At one point during this level, the team would have made a trip to Ray's Occult Books, which was Ray Stantz's book store in Ghostbusters II. A Dopey Dog float was supposed to be the end level boss, but was replaced with a different character. Dopey Dog was a character from The Real Ghostbusters cartoon show.

There was also a "Crowd" mechanic developed for use primarily with this level that was also cut. The "Crowd" mechanic was supposed to simulate the many people that walk the streets of New York City. This "Crowd" mechanic gave up to 1,000 individual NPCs their own AI. The members of the Crowd Mechanic successfully reacted to the Ghostbusters walking by, became scared of ghosts that appeared out of nowhere, and ran in terror when the Stay Puft Marshmallow Man appeared on the street.

There is a visual error in the intro cinematic. Peter Venkman is shown with his Proton Pack Straps without a Proton Pack. In the next scene, the straps are gone.

On the Stay Puft level of the Wii version, there is a poster for a Super Mario Bros. parody game called "Fungus Dudes". This is a reference to the Mushroom Men series. Red Fly developed both Mushroom Men and the Wii version of Ghostbusters: The Video Game.

In the upper floor of the firehouse, there is a computer displaying the infamous ending screen to the NES Ghostbusters game.

In the basement of the firehouse, there is a PKE Meter from The Real Ghostbusters cartoon show and a Ghost Trap from the Extreme Ghostbusters cartoon show.