Despite the character designs being from the 2007 Animated Film, the game uses the voice actors from the 2003 Series by 4Kids Entertainment.

Three Rabbids from the Raving Rabbids series, a Ubisoft property, are guest characters exclusive to the Wii version of the game. One is a generic Raving Rabbid, a new Rabbid variant named Ninja Rabbid that is a parody of the Teenage Mutant Ninja Turtles, and the last, named Splinter Rabbid, is a Rabbid variant from the first two Raving Rabbids games who is a parody of Sam Fisher, the protagonist of Tom Clancy's Splinter Cell series, another Ubisoft property.