Star Fox Adventures notably takes place eight years after the events of Star Fox 64. In an interview with Nintendo Official Magazine in 2002, Takaya Imamura admitted that he felt it'd be better for Fox and his friends to grow and change over time, as opposed to other Nintendo characters like Mario and Bowser who never age.

Imamura would touch upon this again in an 2002 Nintendo Dream interview regarding Star Fox Adventures. In addition to wanting to come up with a justification for why Fox, Falco, Peppy and Slippy would reunite to fight the enemy, he also wanted to reflect the change in genre from SF64 to SFA and how the relationships between the characters had evolved since then.

As mentioned in the same interview, Imamura had already stated some years prior he'd like to make a sequel to Star Fox 64 taking place 20 years later (Fox would be 38 years old) in the official Japanese guidebook for Star Fox 64 released in 1997.

On the Japanese website for Star Fox 64, Slippy mentions certain background characters who would appear in future installments:

• Peppy's wife, Vivian Hare. According to Slippy, Peppy took her to planet Zoness for their honeymoon, which explains why Peppy is so distraught at its polluted status in SF64. Vivian would appear in-game for the first time in Star Fox Command, where it's revealed that she and Peppy had a daughter named Lucy, and that Vivian had died of a disease several years prior.

• Slippy's father, Beltino Toad. According to Slippy, Beltino works as an engineer for Space Dynamics, the manufacturer of the Arwing and many of the other vehicles the Star Fox team utilize. Beltino would later appear in-game for the first time in Star Fox Assault, and would later appear in Star Fox Command.

It's worth pointing that unlike Vivian, Beltino was mentioned in western ancillary SF64 material, that being both the official western Star Fox 64 website and the Star Fox 64 Official Player's Guide, where it states that he helped his son Slippy create the Blue Marine.

Much like the original Star Fox on Super Nintendo, Star Fox 64's stages are split into three difficulty routes: Easy, Normal and Hard. However, unlike the original, SF64 only features two versions of Venom, one for the Easy Route and one for the Hard Route, the latter of which is where the Star Fox team has a final rematch with their rivals, Star Wolf.

It turns out the development team had planned on including another variation of Venom for the Normal route, where Fox McCloud would jump out of his Arwing and battle Andross on-foot with a bazooka. According to the staff, this was scrapped due to time constraints. The pilot mode option seen in Star Fox 64's battle mode is actually the repurposed remnants of this idea.

The green planet Peppy's Arwing is shown hurdling towards in the prologue for Star Fox 64 (and also its 3DS remake, Star Fox 64 3D) is actually meant to represent Papetoon, his and also Fox and James' home planet. The Japanese prologue even refers to it as such, and various Japanese SF64 material also mention it. However, when Star Fox 64 was localized for Western audiences, Nintendo of America completely wiped out all specific mention of Papetoon in both the prologue and also manuals/guidebooks.

According to Takaya Imamura in a 2011 Nintendo Dream magazine interview, Fox McCloud's mother is actually still alive and well on their home planet of Papetoon. This is in contrast with the 1994 Nintendo Power comic series, where it was revealed that Fox's mother (named "Vixy Reinard") was accidentally killed by a car bomb planted by Andross that was intended for her husband ("James" as he would referred to in the games starting with Star Fox 64).

According to Imamura, Fox's mother disapproved of her son following in her husband's footsteps and potentially getting himself killed the same way, and thus they have become estranged from each other, though they haven't exactly broken off from each other completely. Imamura even hints that she's secretly supporting her son's actions from behind the scenes.

One of Peppy's most well known lines, "Never give up. Trust your instincts.", was added because one of the developers, Mitsuhiro Takano, wasn't very good at the game and wanted to get some encouragement.

The developers of the game were originally going to have Fox say "I guess it's your turn to be thankful" to make him sound cooler, but felt that the line was too showy for him. They instead gave the phrase to Falco, who says it after saving Fox from an enemy.

In the Japanese Versions of the game, after being shot down one of Fox's Teammates will shout out his name, similar to Star Fox Assault, but in the Western Versions, they instead shout "No!!!".

The game's opening features the phrase "Star Fox in Nintendo 64". This was a translation error and is supposed to read "Star Fox on Nintendo 64". This was fixed in the 3DS remake.

In the European version, there is an extra language called "Lylat", a made up language which is similar to the gibberish spoken in the original Star Fox.

In the Japanese version, the character icons do not sync with their voices and simply animate the whole way. This was changed in the western versions of the game where the icons would sync with what the characters are saying.

If the player takes too long to defeat the Golemech boss at the end of Venom's easy path (about seven minutes), the fight will end and the player will run into a wall, killing themselves and making the fight restart.

In the Macbeth level, there is a time limit to fighting the boss even if the player missed the Supply Depot ending that leads to Area 6. If the player gets far enough, the boss will state that the player was too slow, and dive bomb the player, killing them instantly. If using a cheat to have infinite health, then the boss will continue to bounce the player upwards until the game crashes.

On the title screen, you can use the analog stick to move the "64" part of the logo around. Fox and his crew will watch it as it moves.

The planet of Katina is a clear reference to the movie "Independence Day". Similar to the movie, you have to dog fight aliens that are invading the world and if you do not finish off the mother ship in time, it will destroy the base with a blue laser. The new partner you get in this fight, Bill Grey, is a reference to the General William Grey from the movie.

The way the characters in the game talk by opening and closing their mouths was inspired by Thunderbirds and other "English puppet dramas" (called "Supermarionation" by Thunderbirds co-creator Gerry Anderson). Shigeru Miyamoto was a fan of these shows and imagined that if the Star Fox series became popular enough, AP Films themselves would come from England to talk about turning it into a "puppet drama".

After obtaining a gold medal on all levels, Expert mode will be unlocked. In Expert mode, Fox wears his father James McCloud's glasses.