Tofu from the secret game mode Tofu Survivor was originally created as a means to test the game's collision detection during development. He later got his own mode as a parody of HUNK's 4th Survivor.

There is an easter egg that allows you to shoot bullet holes in the screen anytime your character is facing the camera.

Originally, the female lead character was supposed to be Elza Walker. When Resident Evil 2 was made again, she was replaced by Claire Redfield. Elza's only appearance was in Resident Evil 1.5.

The S.T.A.R.S. office is way too small for a team of 12 and for some reason only has the desks of Barry, Chris, Jill, Rebecca and Wesker. They may have made some changes after the Mansion Incident but Brad's desk is missing and he had still been alive at the time the office was last used.

The renowned director of zombie movies, George A. Romero, made the original live-action commercials for Resident Evil 2. He was later supposed to direct the live-action Resident Evil movies, but the deal was given to Paul W.S. Anderson.

Normally, when the player searches the leftmost desk in the STARS office (which belongs to Albert Wesker), the player character will remark that the desk has been searched by someone else already. Should the player interact with it 50 times in a row, however, a roll of film can be obtained that, when developed, shows a photo of Rebecca Chambers from the previous game, wearing a basketball outfit. The picture is labelled "Recruit."