When Pokémon Black 2 and Pokémon White 2 first released in Japan, fake leaks were posted on various websites claiming to be ROMs of the game, but in reality contained an FMV of a parody of the theme song to the Disney Channel series Cory in the House. The parody featured several of the games' characters and other memes at the time superimposed over the actors' heads, including talk show host Stephen Colbert, a caricature of the Pokémon fan website Serebii.net with a Trollface superimposed over it, and legacy Pokémon character Gary Oak. Before the title card of the show appears at the end, a barely visible screenshot of a post from the imageboard 4chan can be seen featuring the box art of the Nintendo DS game adaptation of the show, implying that the parody and fake ROMs originated from there. The video also features several scrolling text statements including:

• "Amber killed Gary" - supposedly referring to an incident on 4chan's dedicated Pokémon board /vp/ starting on August 26, 2010 where several posters using Gary Oak and Mewtwo as avatars tried to "revive" or "kill" the anime-only character Amber from The Uncut Story of Mewtwo's Origin by trying to predict their post numbers in advance.
• "SHE IS BLACK ?!?" - referring to Pokémon Black & White Gym Leader Lenora.
• "GIANT HAIR EVERYWHERE" - on the following shot with four Bouffalants superimposed over Mount Rushmore.
• "FINAL GYM LEADER (IT HAS BEEN SEREBII CONFIRMED)" - referring to Iris, the last Gym Leader in Pokémon White and seems to be referring to leaks from the first Pokémon Black and Pokémon White games.
• "THIS FLOATING TEXT IS UP IN YO' SCREEN DAWG"
• "AMERICA" - on a shot of Stephen Colbert.

An upload of the fake ROM's FMV on YouTube dates back to September 16, 2010, two days before Pokemon Black & White's release date in Japan, and features several comments mentioning 4chan and /vp/ on top of the references in the video, suggesting that the hoax actually dates back to the release of the first Pokémon Black and Pokémon White, although discussion of this FMV from that time does not appear to have been preserved. While this ROM is also not known to be preserved (though its contents are), as recently as 2018 there have been reports of ROM dumps of Pokémon White 2 being silently modified to include Cory in the House references, including NPCs with dialogue featuring the lyrics to the show's theme song.

In Generation V, the sounds that play with each individual hit of the move Thrash sound extremely similar to the opening notes of the "Ground Theme" from Super Mario Bros.

If the player character is male and goes to the Rondez-View Ferris Wheel in Nimbasa City in an autumn month, they may battle an NPC named "Nursery Aide Enatsu" (June in English). In the Japanese versions of the game, June is actually a man disguised as a woman in order to secure a job as a Nursery Aide.

Cheren is the first rival to become a Gym Leader within a generation. It took the passing of a full generation for Blue to become a Gym Leader.

In the files for the Pokemon World Tournament, there are two complete text strings for Hilbert and Hilda, the player characters of Black and White. This suggests that they were originally going to be present in an unidentified tournament, and they were also going to speak in full sentences, unlike Red who just speaks in ellipses. Their names are set as Hilbert and Hilda, unlike the Memory Link referencing your past character.

Similar to Pokemon Diamond, Pearl, Platinum, HeartGold, and SoulSilver, Diamond Dust appears in-game. It appears in Iccirus City and Route 8 on various dates, including December 31st. Seeing the dust earns you a medal on your next visit to the Medal Master.

Despite Route 10 being removed from the game, its unique track can still be heard through legitimate means. Visiting Icirrus City during Winter and speaking to the NPC who claims to be the sound designer (located in the ex-Rocket Grunt's house) will change the background music to Route 10's theme.

There's a female trainer named "Guitarist Billy Jo," whose name and appearance resembles a female version of Billie Joe Armstrong, guitarist and singer of the band Green Day.

There's a Lady in Humilau City that says "This dress is comfy and easy to wear," referencing a line from the original Pokemon games where Youngster Ben says "Hi! I like shorts! They're comfy and easy to wear."

Remnants of Route 10, removed from the map in Black 2 & White 2, are still visible in the game's code. The textures, models, and collisions remain, but the scripts, text, and wild Pokemon data are not present.

In Pokestar Studios, there is a specific place where you can surf on the base of a tree. While this is just a glitch and serves no use, it is reminiscent of a multitude of similar surfing glitches in Pokemon Red & Blue.