There was originally going to be a necromorph called the "Flytrap", but the enemy was scrapped sometime during development. It was going to be both an environmental hazard and a regular enemy, intended to encourage the player to slow down, without feeling like an artificial gameplay mechanic. Both of its hands and feet would be stuck to a ceiling, and the skin on its stomach and chest is missing. It would have used its internal organs as a dangling lure, and attacked the player when they got close.

In Chapter 5, if Isaac lets Spoiler: the Tormenter kill him in space, a hand will float through space, as an homage to the original Dead Space cover.

In Chapter 1 in the area with the first shop, there is a working scale that shows Isaac's weight at 195 pounds. After he equips his Engineering Suit, he weighs 255 pounds.

In Chapter 1, the faces of the other marker test subjects on the computer in the observation room, are actually members of the UI Development team.