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Mega Man's up-tilt, the Mega Upper, is from his Marvel vs. Capcom moveset. However, it originated from Mega Man 2: The Power Fighters.
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Donkey Kong's audience cheer in Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate is a reference to the DK Rap from Donkey Kong 64 in chant form.
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When frequently using Little Mac's taunts, there's a slight chance that Doc Louis can be heard giving some advice. Each taunt has a unique sound clip.
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Attachment Mario's blue and pink alternate outfit is based on his appearance in the original Mario Bros. arcade game.
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Attachment One of Princess Zelda's alternate outfits in Super Smash Bros. Brawl and Super Smash Bros. for Nintendo 3DS and Wii U is the same as the design used in Super Smash Bros. Melee, based on her appearance in The Legend of Zelda: Ocarina of Time.
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Attachment Two of Zero Suit Samus' alternate costumes in Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate are based off of the perfect ending in Metroid: Zero Mission.
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Attachment One of Little Mac's alternate costumes in Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate is a reference to the Punch-Out!! arcade game, where he was represented by a translucent array of green lines.
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subdirectory_arrow_right Mario (Franchise), Mario Kart (Collection)
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In the Mario Kart games, Rosalina is a heavy/large class character. However, in the Super Smash Bros. series she is a light class character. The reason she is a heavy class character in Mario Kart is most likely due to her height.
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Attachment One of Little Mac's palette swaps in Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate is based on his appearance in Super Punch-Out!!.
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In Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate, Kirby's dark blue alternate color with yellow eyes is designed after Meta Knight's appearance without a mask. This is based on Meta Knight's appearance after being defeated in Kirby's Adventure.
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Attachment One of Mario's costumes in Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate is the patriotic Mario skin from NES Open Tournament Golf released in 1991.
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Attachment One of Luigi's alternate costumes is based on his appearance in the Japan-only movie, Super Mario Bros.: Peach-hime Kyushutsu Dai Sakusen!.
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Attachment When Kirby takes Zelda's ability, he wears the headdress Zelda wears as a child in Ocarina of Time, despite the fact Zelda is an adult in the Super Smash Bros. series and the headdress appears nowhere else in the game.

Super Smash Bros. Ultimate updates this appearance to use Zelda's hair instead.
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Since Super Smash Bros. Brawl, Wario is the only character in the series to have a 4th taunt. It is activated by pushing any of the taunt buttons while he is riding the Wario Bike.
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In the Japanese versions of Super Smash Bros. and Super Smash Bros. Melee, the Beam Sword sounded similar to a Lightsaber from the Star Wars series. This was changed during localization for other regions. In Super Smash Bros. Brawl onwards, the sound effects have been kept the same for all versions.
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Attachment When selecting the Famicom and NES color schemes for R.O.B., the stickers on their base change to reflect their real-life counterparts. The decal for the Japanese Famicom R.O.B. reads "Family Computer Robot", while the decal for the North American NES R.O.B. reads "Robotic Operating Buddy".

In Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate, these regional color schemes based on R.O.B.'s original models for the Famicom and NES are made his default palette depending on if you are playing the Japanese or International versions of the games respectively.
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Attachment It's possible that the blue outfit for R.O.B. is based on his cameo as Nav-Com in Star-Tropics for the NES.
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In all of Captain Falcon's appearances within the Super Smash Bros. series, his red color swap is actually the outfit of Blood Falcon, his evil clone in the F-Zero games, complete with the correct insignia.
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Aside from Pikachu and Jigglypuff (whose costume accessories change from game to game), Samus is the only character to have one of her team-based costumes change between games: In the original Smash Bros. and Melee, Samus wears her default (orange) costume when she is on the red team, but in Brawl, her pink costume is used instead. This could be due to the fact that in her Zero Suit, her default costume color is blue. So they needed to give her a new costume that would depict both forms in something closely resembling Red.
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Pikachu is the only playable Pokémon in the Super Smash Bros. series to have a visible gender difference in the Pokémon series. Male Pikachus look like the one in Smash Bros, but females have a small indent in their tail, which vaguely looks like a heart. However, gender differences in Pokémon were introduced in Generation IV, which came out after the first Super Smash Bros. and Melee.

Female variants were later included in Super Smash Bros. Ultimate.
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