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Peach's Final Smash since Super Smash Bros. Brawl is a piece of music from Super Mario Bros 3 that has been sped up. The music is from World 5: The Sky, Coin Heaven bonus levels and in the Warp Zone.
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Mario's Cape attack can be used on a taunting character to make them face the other way in ways that are otherwise impossible, like making Wario face the screen while doing his side taunt.
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All lines spoken by Marth in Melee/Brawl/Wii U and 3DS are in Japanese. These are what they all are translated:

Marth's taunt - "Everyone, please watch me!" ("みんな、見ていてくれ!")
Marth's counter - "There!" ("そこだ!"), "I won't let you!" ("させるものか!")
Marth's victory poses - "This time, victory is mine, right?" ("今回は僕の勝ちだね。"), "Today, too, I managed to survive." ("今日も生き延びることが出来た。"), "There's no way I can lose!" ("僕は負ける訳には行かないんだ。")

He also has an unused sound file in the Debug Menu of the game which is "Let's Dance!" ("レッツダンス!")
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Attachment Snake's alternate palette swaps in Super Smash Bros. Brawl and Super Smash Bros. Ultimate are based on wearable camouflage outfits in Metal Gear Solid 3, such as the Fire camo and Spirit camo.
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In all of her Smash Bros. appearances, Peach is depicted with a ring on her middle finger, which is absent in other games.
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Attachment Jigglypuff's eyes are blue in the Pokemon series, but green in the first three games of the Super Smash Bros. series, the same color as a shiny Jigglypuff.
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In SSB and Melee the first syllable of "Samus" is pronounced /sɑ/ ("saw"). However, in Brawl, it's pronounced /sæ/ ("sah"). This change in pronunciation is consistent with the way Samus is pronounced in Metroid Prime 3: Corruption.
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The following fighters are the only characters in the Smash series to appear in another fighting game:

•Snake appeared in Dream-Mix TV World Fighters as a playable character.
•Mega Man has appeared in the Mega Man Power Battle series, a few Capcom VS games (mainly the Marvel vs. Capcom series) and Street Fighter X Tekken (in his "Bad Box Art" form).
•Donkey Kong also appeared in Punch-Out!! for Wii as a boss.
•Sonic appeared as a playable character in Sonic the Fighters and Sonic Battle.
•Link appeared in the GameCube version of SoulCalibur II.
•Little Mac appears in Punch-Out!!.
•Ryu and Ken are main characters from the Street Fighter series.
•Cloud appears as a playable character in the Dissidia Final Fantasy series.
•Terry is a main character from The King of Fighters series.
•Kazuya is one of the characters in the Tekken series.
•The Kirby series has its own set of spin-off fighting games called Kirby Fighters.
•Charizard, Lucario, Mewtwo and Pikachu appear in the fighting game Pokken Tournament.
•Min Min is one of the characters in ARMS.
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Pikachu is the only character in the entire Super Smash Bros series to only have four costume color options in the first three games.
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Kirby uses a different dash attack in each of the first three Super Smash Bros. games. In the original Super Smash Bros., he performs a slide tackle. In Melee and Ultimate, he performs "Burning Kirby" (an ability seen in some Kirby games, and merged with the Fire ability in others), while in Brawl and Smash 4, he performs Yo-Yo Kirby's "Break Spin" from Kirby Super Star.
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Pokémon Trainer is the only character that has a custom name in their original series to not be given a default name in Brawl, and is instead given a generic name. It is clear from the character design that the suitable default name would be "Red" as he is named as such in Pokémon Gold and Silver, and again in the remakes which were released after Brawl. The reason this was done is unknown.
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Pikachu is the only playable Pokémon in the Super Smash Bros. series to have a visible gender difference in the Pokémon series. Male Pikachus look like the one in Smash Bros, but females have a small indent in their tail, which vaguely looks like a heart. However, gender differences in Pokémon were introduced in Generation IV, which came out after the first Super Smash Bros. and Melee.

Female variants were later included in Super Smash Bros. Ultimate.
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Aside from Pikachu and Jigglypuff (whose costume accessories change from game to game), Samus is the only character to have one of her team-based costumes change between games: In the original Smash Bros. and Melee, Samus wears her default (orange) costume when she is on the red team, but in Brawl, her pink costume is used instead. This could be due to the fact that in her Zero Suit, her default costume color is blue. So they needed to give her a new costume that would depict both forms in something closely resembling Red.
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In all of Captain Falcon's appearances within the Super Smash Bros. series, his red color swap is actually the outfit of Blood Falcon, his evil clone in the F-Zero games, complete with the correct insignia.
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Attachment It's possible that the blue outfit for R.O.B. is based on his cameo as Nav-Com in Star-Tropics for the NES.
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Attachment When selecting the Famicom and NES color schemes for R.O.B., the stickers on their base change to reflect their real-life counterparts. The decal for the Japanese Famicom R.O.B. reads "Family Computer Robot", while the decal for the North American NES R.O.B. reads "Robotic Operating Buddy".

In Super Smash Bros. for Nintendo 3DS and Wii U and Super Smash Bros. Ultimate, these regional color schemes based on R.O.B.'s original models for the Famicom and NES are made his default palette depending on if you are playing the Japanese or International versions of the games respectively.
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In the Japanese versions of Super Smash Bros and Super Smash Bros: Melee, the Beam Sword sounded similar to a Lightsaber from the Star Wars series. This was changed during localization for other regions. In Super Smash Bros: Brawl, the sound effects have been kept the same for all versions.
subdirectory_arrow_right Super Smash Bros. Melee (Game)
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Attachment Although he appears to be a flat sprite, Mr. Game & Watch is actually a 3D model designed to look 2D from the perspective of the camera.
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Since Super Smash Bros. Brawl, Wario is the only character in the series to have a 4th taunt. It is activated by pushing any of the taunt buttons while he is riding the Wario Bike.
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Attachment When Kirby takes Zelda's ability, he wears the headdress Zelda wears as a child in Ocarina of Time, despite the fact Zelda is an adult in Super Smash Bros and the headdress appears nowhere else in the game.

Super Smash Bros. Ultimate updates this appearance to use Zelda's hair instead.
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