Strider Hiryu will exclaim an audible "Hah!" every time he swings his cypher in the Japanese release. This was removed from the international release.

The sprite for the Amazon enemy has its left breast exposed, but when rendered in game, a separate sprite of a leaf is placed over it.

In the game's third stage Flying Battleship Balrog, in the upper-leftmost corner of the first room inside the ship are two stacks of artillery shells. Destroying the shells will reveal a panda that dances briefly before disappearing. According to Strider's designer and director Kouichi Yotsui, this easter egg was snuck in without his knowledge as a joke, and the person who did this later became the wife of Mega Man character designer and later series producer Keiji Inafune.