One of the power-ups in New Super Chick Sisters is the "Sea Kitten Suit", a reference to an unsuccessful PETA campaign from around the same time advocating for people to begin calling fish "sea kittens" so people would be put off from the idea of eating a "kitten".

New Super Chick Sisters contains an alternate "kid-friendly" mode. Despite what the name may imply, it does not alter the game's difficulty, nor does it remove the game's excessive use of blood, dialogue from NPCs graphically describing slaughter of chickens, or a line of dialog from Bowser namedropping the pornographic Pamella Anderson movie Barb Wire. What is does do, however, is remove a graphic real-world slaughterhouse video played midway through the game.