To promote the release of Astro Bot, its predecessor Astro's Playroom received a free content update on July 7, 2024. This update added new artifacts to the Gatcha machine based on various PlayStation 5 accessories (such as the PlayStation VR2 headset and the PlayStation Portal) that could be accessed through a new room in the PlayStation Labo, and a brand new mission room that displayed the countdown to Astro Bot's release.

Additionally, four new Bots were added to be rescued, one for each world and each one based on a PlayStation character. The Bots in question were based on Lady Maria of the Astral Clocktower from Bloodborne, Selene Vassos from Returnal, a racer from the Gran Turismo series, and a Pipo Monkey from the Ape Escape series. The four Bots collected in Astro's Playroom could then be transferred to the starting crew of Astro Bot, where they would respectively unlock the V.I.P. Bots "Nightmare Guardian", "Stranded Scout", "Pro Driver", and "Greedy Ape".